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Methods Animals Animal experiments were performed with adherence to all national and local guidelines and regulations, the Guide for the Care and Use of Laboratory Animals 25 , and the ARRIVE guidelines, and with the approval of the Institutional Animal Care and Use Committee at The University of Texas Medical Branch (UTMB)

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Sufficient NAD+ availability is necessary for these enzymes to operate, so raising NAD+ levels could in theory modulate pathways related to aging, inflammation, and cell survival

Improved Cognitive Function B12 supports brain health and cognitive performance through several mechanisms: Maintains myelin sheaths that protect and insulate nerves Supports neurotransmitter production and regulation Enhances blood flow and oxygen delivery to the brain Reduces homocysteine levels (elevated levels are linked to cognitive decline) Many clients report better concentration, improved memory, and greater mental clarity after beginning B12 therapy